Pressure acceleration of proteolysis: A general mechanism

نویسندگان

  • Kazuyuki Akasaka
  • Harumi Nagahata
  • Akihiro Maeno
  • Ken Sasaki
چکیده

Remarkable acceleration of enzymatic proteolysis by pressure at kbar range is reported with ubiquitin as substrate and α-chymotrypsin as enzyme. The acceleration is interpreted in terms of the shift of conformational equilibrium in ubiquitin from the non-degradable folded conformer to the enzyme-degradable unfolded conformer by pressure because of the lower volume of the latter, while the enzymatic activity of α-chymotrypsin is still largely retained. This mechanism is considered generally applicable to most globular proteins and the method of pressure-accelerated proteolysis will have an enormous potential utility in systems wherever efficient removal of proteins is needed.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2008